Cell culture

Use the C-Netics to monitor any suspension cell culture

Cell culture is a commonly used process  in the biopharmaceutical industries for biological compounds production such as antibodies, hormones or enzymes. Cells are cultured so that they multiply and create large amounts of molecules of interest that are harvested at the end of the process. Those molecules will be key active ingredients for the final drug manufacturing.

Cultured cells are also required in the manufacturing of some Advanced Therapy Medicinal Products (ATMPs). ATMPs are medicines for human use, produced through biological process including cell therapy, gene therapy and cell engineered products. These groundbreaking drugs are revolutionizing medicine by providing new opportunities for treating cancers, degenerative diseases and genetic disorders. 

Cell Therapy is a promising treatment against some types of cancer as the cells are genetically modified in order to selectively target and kill cancer cells, thus reducing the well known side effects caused by classic treatments, such as chemotherapy. 

Cell therapy a promising example of ATMP


For some types of therapies, cells are taken directly from the patient, for others they are purchased from a supplier.


Cells of interest are isolated by different lab processes.

03. Reprogram

Cells are genetically modified to better fight the patient’s disease.

Cell expansion

Cells multiply in a bioreactor for several weeks. 


Cells or molecules produced by cells are injected in the patient to treat him.

Overcoming the challenges related to real-time monitoring during cell expansion

To successfully grow cells, an appropriate artificial environment has to be created. Different parameters need to be monitored to ensure optimal cell expansion, such as their viability and density. This is a time-consuming and delicate operation requiring multiple manual steps, significantly increasing contamination risks.
Culture monitoring requires to collect cell culture samples on a regular basis. Then, cell’s state can be determined based on the physical changes occurring during cell death. Finally, data are analyzed and post-processed to get a numerical values for each parameter of interest.

These three steps represent many significant challenges: first, the analyzed sample must be representative of the whole cell culture to ensure accuracy and precision, independently on the culture volume. Sampling must be done regularly to follow culture’s health trend. However, sampling leads to important contamination risk as the main container need to be opened to take samples.

The C-Netics overcomes the previously highlighted challenges as it is able to measure cell viability, density, culture medium pH and other metabolites present in cell cultures in a label-free manner, thanks to microelectrodes in a single-use cartridge.